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Mondal, Keshab Chandra
- Antibacterial, Anti-Diabetic and Anti-Inflammatory Property of the Sea Weed, Porteresia coarctata, Collected from Mangrove Fringed Mudflats of Sundarban Coast, West Bengal
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Authors
Affiliations
1 Department of Microbiology, Panskura Banamali College, Midnapur (East) 721152, West Bengal, IN
2 Department of Microbiology, Vidyasagar University, Midnapur (West) 721102, West Bengal, IN
3 Department of Marine Science, University of Calcutta, 35, B.C. Road, Kolkata-700019, West Bengal, IN
1 Department of Microbiology, Panskura Banamali College, Midnapur (East) 721152, West Bengal, IN
2 Department of Microbiology, Vidyasagar University, Midnapur (West) 721102, West Bengal, IN
3 Department of Marine Science, University of Calcutta, 35, B.C. Road, Kolkata-700019, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 14, No Spl (2017), Pagination: 4-5Abstract
Porteresia coarctata (= Oryza coarctata) is a perennial halophytic wild grass, relative of rice and member of the family Poaceae. It acts as a pioneer species in the succession process of mangrove formation along the estuaries of India. The sequestering carbon, fertilizer in aquaculture and salt tolerant property of this mangrove associate has been dealt with by a number of workers earlier. But, the present study was conducted to evaluate the antibacterial property of aqueous, acetone, ethanol and methanol extracts of Porteresia coarctata collected from the Matla river of Indian Sunderban delta. Collected sea weeds were screened for their antibacterial studies against gram positive bacteria including Staphylococcus aureus, Streptococcus fecalis and Bacillus subtilis and gram-negative bacteria including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonia and Proteus vulgaris using disc diffusion method. Present study shows that ethanol extracts of Porteresia coarctata has maximum antibacterial activity against E.coli (1.2±0.01 mm) and Streptococcus fecalis (1.4±0.01 mm) at an MIC of 700 μg/mL and 500 μg/mL, respectively. Along with the antimicrobial activities, seaweeds also showed anti-diabetic activity and but have no anti-inflammatory activity. Therefore, the results suggested that these sea weeds could be exploited in the management of various infectious diseases and can be used as for pharmaceutical purpose.- Enhanced Production of α-Amylase from Bacillus licheniformis SKB 4
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Authors
Saptadip Samanta
1,
Sanjay Kar
2,
Pradeep Kumar Das Mohapatra
3,
Bikash Ranjan Pati
3,
Keshab Chandra Mondal
3
Affiliations
1 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
2 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
3 Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, IN
1 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
2 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur 721101, West Bengal, IN
3 Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, IN
Source
Indian Science Cruiser, Vol 31, No 5 (2017), Pagination: 32-39Abstract
The effect of some inductive factors including incubation temperature, aeration, inorganic phosphate source, supplementation of amino acids and vitamins on production of extracellular amylase by Bacillus licheniformis SKB 4 was optimized in a submerged fermentation. The maximum production of enzyme was obtained in highly aerobic condition at 42°C, pH 6.5 and 24 h incubation period. 1%, v/v inoculumn concentration exerts best result for growth and enzyme production. Several chemical factors such as inorganic phosphate (KH2PO4; 0.3%), amino acids (0.5 mg %; glutamic acid, cysteine, isoleucine), vitamins (biotin, 50μg/ml; pyridoxine, 50μg/ml; riboflavin, 20μg/ml), metallic salt MgSO4 (1 mM) and surfactant Tween 80 (0.075% (v/v) have positive effect on amylase production. Among the raw food grains used for amylase production, soya bean seed favoured enzyme production and rate production is much higher (2.5 fold) than the normal culture medium, whereas cereals and other pulses have no effect on enzyme production within normal incubation time. Use of raw food grain as culture medium and optimization of certain factors are the most important part for production of amylase in industrial sector.Keywords
Inductive Factors of Amylase Production from Bacillus licheniformis SKB4.References
- R Gupta, P Gigras, H Mohapatra, V K Goswami and B Chauhan, Microbial μ-amylases: a biotechnological perspective, Process Biochem, 38, 1599–1616, 2003.
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- B K Lonsane, M V Ramesh, Production of bacterial thermostable alpha-amylase by solid-state fermentation: a potential tool for achieving economy in enzyme production and starch hydrolysis, Adv Appl Microbiol, 35, 1-56, 1990.
- B Kar, R Banerjee and B C Bhattacharyya, Optimization of physicochemical parameters for gallic acid production by evolutionary operation-factorial design technique, Proc. Biochem, 37, 1395-1401, 2002.
- K K Prasad, S V Mohan, R S Rao, B R Pati and P N Sarma, Production by Pleurotus ostreatus 1804; Optimization of submerged culture conditions by Taguchi DOE methodology, Biochem. Eng. J, 24, 17-26, 2005.
- S Samanta, M Jana, C Maity, S Kar, P K D Mohapatra, B R Pati and KC Mondal, The potential of immobilized bacterial μ-amylase on coconut coir, a smart carrier for biocatalysts, Biocat and Biotransform, 27(2), 131-135, 2009.
- P Bernfeld, Amylases alpha/beta, Methods in enzymology, 1, 149-158, 1955.
- M S Tanyildizi, D Ozer and M Elibol, Optimization of α-amylase production by Bacillus sp. using response surface methodology, Process Biochem, 40, 2291–2296, 2005.
- S Samanta, A Das, S K Halder, A Jana, S Kar, P K D Mahapatra, B R Pati and K C Mondal, Thermodynamic and kinetic characteristics of an μ-amylase from Bacillus licheniformis SKB4, Acta Biolgica Szegediensis, 58(2), 147-156, 2014.
- P Deb, S A Talukdar, K Mohsina, P K Sarker and S M Abu Sayem, Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciens P-001, Springerplus, 2: 154, 2013.
- L Rasooli, S D A Astaneh, H Borna and K A Barchini, A thermostable μ-amylase producing natural variant of Bacillus sp. (B. licheniformis) isolated from soil in Iran, Am. J. Agri. and Biol. Sci, 3, 591-596, 2008.
- S Aiba, K Kitai and T Imanaka, Cloning and expression of thermostable μ-amylase gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis. Appl Environ Microbiol, 46, 1059-1065, 1983.
- S Mahmood and S R Rahman, Production and Partial Characterization of Extracellular μ-Amylase by Trichoderma viride, Bangladesh J Microbiol, , 25(2), 99-103, 2008.
- R D Wind, R M Buitelaar, G Eggink, H J Huizing and L Dijkhvizen, Characterization of a new Bacillus stearothermophilus isolate; a highly thermostable α-amylase producing strain, Appl. Microbiol. Biotechnol, 41, 155162, 1994.
- N Saito and K Yamamoto, Regulatory factors affecting μ-amylase production in Bacillus licheniformis, J. Bacteriol, 121, 848-856, 1975.
- M E Haddad, I M Gracheva and S V Gryaznova, Effect of homogeneity of the culture, age and inoculum size on the biosynthesis of amylolytic enzymes by Bacillus mesentericus PB, Microbiologia, 10, 365-368, 1974.
- Z Baysal, F Uyar and C Ayteki, Production of μ-amylase by thermotolerant Bacillus subtilis in the presence of some carbon, nitrogen containing compounds and surfactants, Annals of Microbiol, 53, 323-328, 2003.
- M Thirunavukkarasu and F G Priest, Regulation of amylase synthesis in Bacillus licheniformis NCIB 6346, Biotechnol. Lett, 7, 315- 318, 1980.
- N Akcan, High Level Production of Extracellular μ-Amylase from Bacillus licheniformis ATCC 12759 in Submerged Fermentation, Romanian Biotechnological Lett, 16(6), 2011.
- S Sen and S L Chakrabarty, Amylase from Lactobacillus cellobiosus isolated from vegetable wastes, J. Ferment. Technol, 62, 407-413, 1984.
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- H K Sodhi, K Sharma, J K Gupta and S K Soni, Production of a thermostable μ-amylase from Bacillus sp. PS-7 by solid-state fermentation and its synergistic use in the hydrolysis of malt starch for alcohol production, Process Biochem, 40, 525–534, 2005.
- N Goyal, J K Gupta and S K Soni, A novel raw starch digesting thermostable μ-amylase from Bacillus sp. I-3 and its use in the direct hydrolysis of raw potato starch. Enz. Microbiol. Technol, 37, 723–734, 2005.
- J M Khire and A Pant, Thermostable, Salttolerant amylase from Bacillus sp. 64, W, J. Microbiol. Biotechnol, 8, 167-170, 1992.
- R Malhotra, S M Noorvez and T Satyanarayana, Production and partial characterization of thermostable and calcium independent alpha amylase of an extreme thermophile Bacillus thermooleovorans NP54, Lett. Appl. Microbiol, 31, 378–384, 2000.
- S Arnesen, S H Eriksen, J Olsen and B Jensen, Increased production of a-amylase from Thermomyces lanuginosus by the addition of Tween 80, Enz. Microbiol. Technol, 23, 249–252, 1989.
- J L U M Rao and T Satyanarayana, Enhanced secretion and low temperature stabilization of a hyperthermostable and Ca2+ independent a-amylase of Geobacillus thermoleovorans by surfactants, Lett. Appl. Microbiol, 36, 191–196, 2003.
- R A K Srivastava and J N Baruah, Culture conditions for production of thermostable amylase by Bacillus stearothermophilus, Appl. Environ. Microbiol, 52, 179-184, 1986.
- Antibacterial, Anti-Diabetic and Anti-Inflammation Property of the Sea Weed, Porteresia coarctata, Collected from Mangrove Fringed Mudflat of Sundarban Coast, West Bengal
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Authors
Affiliations
1 Department of Microbiology, Panskura Banamali College, Midnapur (E)-721152, West Bengal, IN
2 Department of Microbiology, Vidyasagar University, Midnapur (W)-721102, West Bengal, IN
1 Department of Microbiology, Panskura Banamali College, Midnapur (E)-721152, West Bengal, IN
2 Department of Microbiology, Vidyasagar University, Midnapur (W)-721102, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 14, No 2 (2017), Pagination: 193-200Abstract
Porteresia coarctata (Syn = Oryza coarctata) is a perennial halophytic wild grass, relative of rice, member of Poaceae and acts as a pioneer species in the succession process of mangrove formation along the estuaries of India. The sequestering carbon, fertilizer in aquaculture and salt tolerance property of this mangrove associate has been dealt with by a number of workers earlier. But, the present study was to evaluate the antibacterial property of aqueous, acetone, ethanol and methanol extracts of Porteresia coarctata collected from the Matla river of Indian Sundarban delta. Collected sea weeds were screened for their antibacterial studies against gram positive bacteria including Staphylococcus aureus, Streptococcus fecalis and Bacillus subtilis and gram-negative bacteria including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumonia and Proteus vulgaris using disc diffusion method. Present study shows that ethanol extracts of Porteresia coarctata has maximum antibacterial activity against E.coli (1.2±0.01 mm) and Streptococcus fecalis (1.4±0.01 mm) at an MIC of 700 μg/mL and 500 μg/mL, respectively. Along with the antimicrobial activities, seaweeds also showed anti-diabetic activity and but have no anti-inflammation activity. Therefore, the results suggest that these sea weeds could be exploited in the management of various infectious diseases and can be used as for pharmaceutical purpose.Keywords
Porteresia coarctata, Antibacterial, Sundarban, Anti-Diabetic, Anti-Inflammation.References
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- Studies on Regulation of Xylanase Bio-Synthesis by Trichoderma reesei SAF3
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Authors
Affiliations
1 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
2 PG Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, IN
3 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
4 Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, IN
1 Department of Botany, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
2 PG Department of Botany, Ramananda College, Bishnupur, Bankura, 722122, West Bengal, IN
3 Department of Physiology, Midnapore College, Midnapore, Paschim Medinipur, 721101, West Bengal, IN
4 Department of Microbiology, Vidyasagar University, Paschim Medinipur 721102, West Bengal, IN
Source
Indian Science Cruiser, Vol 33, No 3 (2019), Pagination: 19-26Abstract
Microbial xylanases have an exciting potentiality and are being used extensively in different industries. In this study, regulation of xylanase bio-synthesis was examined in Trichoderma reesei SAF3. Xylan (1.0% w/v) showed the highest inductive effect. Glucose at concentration of 1% (w/v) was repressive for xylanase bio-synthesis when used in presence or absences of xylan; the repression was partially elevated by addition of cyclic adenosine monophosphate (cAMP, 5mM). The above findings suggested that xylan was the major inducer and the induction was energy dependent, mediated by second messenger system.Keywords
Xylanase, Regulation of Biosynthesis, cAMP, Trichoderma Reesei.References
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- Effect of Some Heavy Metal (Zn, Cu, Pb) Pollutants and Microbial Load on the Edible Oyster (Saccostrea cucullata) in Sundarban, West Bengal
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Authors
Harekrishna Jana
1,
Bivas Kumar Malik
1,
Puspendu Shit
1,
Partha Pratim Chakraborty
1,
Keshab Chandra Mondal
2
Affiliations
1 Department of Microbiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, IN
2 Department of Microbiology, Vidyasagar University, Paschim Medinipur Pin-721102, West Bengal, IN
1 Department of Microbiology, Raja N. L. Khan Women’s College, Paschim Medinipur, Pin-721102, West Bengal, IN
2 Department of Microbiology, Vidyasagar University, Paschim Medinipur Pin-721102, West Bengal, IN
Source
Journal of Environment and Sociobiology, Vol 18, No 1 (2021), Pagination: 1-13Abstract
Total bacterial count (TBC), total coliform (TC), total faecal coliform (TFC), E. coli and Bifidobacterium sp. in the flesh of edible oyster (Saccostrea cucullata), in three collecting stations of Sundarban, varied during different seasons of a year. The bacterial load was maximum in monsoon and minimum in pre monsoon. Heavy metal content in the flesh showed following sequence: Zn>Cu>Pb, with maximum values during monsoon and minimum values during pre-monsoon. Further, maximum antioxidant enzyme (Catalase and Superoxide dismutase) activities in the oyster were recorded during pre-monsoon which decreased in monsoon, while the level of lipid peroxidation (free radical) was minimum in pre-monsoon. It appears, therefore, that the antioxidant activity in oyster was inversely proportional to the heavy metal concentration in the flesh. Hence, monitoring of heavy metal content in the water of Sundarban estuary is considered vital towards the survivability of the oysters.Keywords
Bacterial Load, Heavy Metals, Antioxidant Enzyme, Oyster, Sundarban.References
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